Poplar trees reconfigure the transcriptome and metabolome in response to drought in a genotype- and time-of-day-dependent manner.
Identifieur interne : 001B92 ( Main/Exploration ); précédent : 001B91; suivant : 001B93Poplar trees reconfigure the transcriptome and metabolome in response to drought in a genotype- and time-of-day-dependent manner.
Auteurs : Erin T. Hamanishi [Canada] ; Genoa L H. Barchet [Canada] ; Rebecca Dauwe [Canada, France] ; Shawn D. Mansfield [Canada] ; Malcolm M. Campbell [Canada]Source :
- BMC genomics [ 1471-2164 ] ; 2015.
Descripteurs français
- KwdFr :
- ARN des plantes (analyse), ARN des plantes (isolement et purification), ARN des plantes (métabolisme), Analyse de regroupements (MeSH), Chromatographie gazeuse-spectrométrie de masse (MeSH), Facteurs temps (MeSH), Génotype (MeSH), Métabolisme énergétique (génétique), Métabolome (MeSH), Populus (génétique), Populus (métabolisme), Réseaux de régulation génique (MeSH), Sécheresses (MeSH), Transcriptome (MeSH).
- MESH :
- analyse : ARN des plantes.
- génétique : Métabolisme énergétique, Populus.
- isolement et purification : ARN des plantes.
- métabolisme : ARN des plantes, Populus.
- Analyse de regroupements, Chromatographie gazeuse-spectrométrie de masse, Facteurs temps, Génotype, Métabolome, Réseaux de régulation génique, Sécheresses, Transcriptome.
English descriptors
- KwdEn :
- Cluster Analysis (MeSH), Droughts (MeSH), Energy Metabolism (genetics), Gas Chromatography-Mass Spectrometry (MeSH), Gene Regulatory Networks (MeSH), Genotype (MeSH), Metabolome (MeSH), Populus (genetics), Populus (metabolism), RNA, Plant (analysis), RNA, Plant (isolation & purification), RNA, Plant (metabolism), Time Factors (MeSH), Transcriptome (MeSH).
- MESH :
- chemical , analysis : RNA, Plant.
- genetics : Energy Metabolism, Populus.
- chemical , isolation & purification : RNA, Plant.
- metabolism : Populus, RNA, Plant.
- Cluster Analysis, Droughts, Gas Chromatography-Mass Spectrometry, Gene Regulatory Networks, Genotype, Metabolome, Time Factors, Transcriptome.
Abstract
BACKGROUND
Drought has a major impact on tree growth and survival. Understanding tree responses to this stress can have important application in both conservation of forest health, and in production forestry. Trees of the genus Populus provide an excellent opportunity to explore the mechanistic underpinnings of forest tree drought responses, given the growing molecular resources that are available for this taxon. Here, foliar tissue of six water-deficit stressed P. balsamifera genotypes was analysed for variation in the metabolome in response to drought and time of day by using an untargeted metabolite profiling technique, gas chromatography/mass-spectrometry (GC/MS).
RESULTS
Significant variation in the metabolome was observed in response the imposition of water-deficit stress. Notably, organic acid intermediates such as succinic and malic acid had lower concentrations in leaves exposed to drought, whereas galactinol and raffinose were found in increased concentrations. A number of metabolites with significant difference in accumulation under water-deficit conditions exhibited intraspecific variation in metabolite accumulation. Large magnitude fold-change accumulation was observed in three of the six genotypes. In order to understand the interaction between the transcriptome and metabolome, an integrated analysis of the drought-responsive transcriptome and the metabolome was performed. One P. balsamifera genotype, AP-1006, demonstrated a lack of congruence between the magnitude of the drought transcriptome response and the magnitude of the metabolome response. More specifically, metabolite profiles in AP-1006 demonstrated the smallest changes in response to water-deficit conditions.
CONCLUSIONS
Pathway analysis of the transcriptome and metabolome revealed specific genotypic responses with respect to primary sugar accumulation, citric acid metabolism, and raffinose family oligosaccharide biosynthesis. The intraspecific variation in the molecular strategies that underpin the responses to drought among genotypes may have an important role in the maintenance of forest health and productivity.
DOI: 10.1186/s12864-015-1535-z
PubMed: 25895923
PubMed Central: PMC4437445
Affiliations:
- Canada, France
- Colombie-Britannique , Hauts-de-France, Ontario, Picardie
- Toronto, Vancouver, cedex
- Université de Toronto, Université de la Colombie-Britannique
Links toward previous steps (curation, corpus...)
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Campbell</name><affiliation wicri:level="4"><nlm:affiliation>Faculty of Forestry, University of Toronto, 33 Willcocks St., Toronto, ON, M5S 3B2, Canada. malcolm.campbell@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Faculty of Forestry, University of Toronto, 33 Willcocks St., Toronto, ON, M5S 3B2</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Centre for the Analysis of Genome Evolution and Function, University of Toronto, 25 Willcocks St., Toronto, ON, M5S 3B2, Canada. malcolm.campbell@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre for the Analysis of Genome Evolution and Function, University of Toronto, 25 Willcocks St., Toronto, ON, M5S 3B2</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Department of Cell & Systems Biology, University of Toronto, 25 Willcocks St., Toronto, ON, M5S 3B2, Canada. malcolm.campbell@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Cell & Systems Biology, University of Toronto, 25 Willcocks St., Toronto, ON, M5S 3B2</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Sciences, University of Toronto Scarborough, Toronto, ON, M1C 1A4, Canada. malcolm.campbell@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biological Sciences, University of Toronto Scarborough, Toronto, ON, M1C 1A4</wicri:regionArea><wicri:noRegion>M1C 1A4</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25895923</idno><idno type="pmid">25895923</idno><idno type="doi">10.1186/s12864-015-1535-z</idno><idno type="pmc">PMC4437445</idno><idno type="wicri:Area/Main/Corpus">001D31</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001D31</idno><idno type="wicri:Area/Main/Curation">001D31</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001D31</idno><idno type="wicri:Area/Main/Exploration">001D31</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Poplar trees reconfigure the transcriptome and metabolome in response to drought in a genotype- and time-of-day-dependent manner.</title><author><name sortKey="Hamanishi, Erin T" sort="Hamanishi, Erin T" uniqKey="Hamanishi E" first="Erin T" last="Hamanishi">Erin T. Hamanishi</name><affiliation wicri:level="4"><nlm:affiliation>Faculty of Forestry, University of Toronto, 33 Willcocks St., Toronto, ON, M5S 3B2, Canada. erin.hamanishi@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Faculty of Forestry, University of Toronto, 33 Willcocks St., Toronto, ON, M5S 3B2</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Centre for the Analysis of Genome Evolution and Function, University of Toronto, 25 Willcocks St., Toronto, ON, M5S 3B2, Canada. erin.hamanishi@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre for the Analysis of Genome Evolution and Function, University of Toronto, 25 Willcocks St., Toronto, ON, M5S 3B2</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Barchet, Genoa L H" sort="Barchet, Genoa L H" uniqKey="Barchet G" first="Genoa L H" last="Barchet">Genoa L H. Barchet</name><affiliation wicri:level="4"><nlm:affiliation>Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada. genoalily@gmail.com.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, BC, V6T 1Z4</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Dauwe, Rebecca" sort="Dauwe, Rebecca" uniqKey="Dauwe R" first="Rebecca" last="Dauwe">Rebecca Dauwe</name><affiliation wicri:level="4"><nlm:affiliation>Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada. rebecca.dauwe@u-picardie.fr.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, BC, V6T 1Z4</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Current address: Département des sciences agronomiques et écologiques, Université de Picardie Jules Verne, Amiens, 80039 cedex, France. rebecca.dauwe@u-picardie.fr.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Current address: Département des sciences agronomiques et écologiques, Université de Picardie Jules Verne, Amiens, 80039 cedex</wicri:regionArea><placeName><region type="region" nuts="2">Hauts-de-France</region><region type="old region" nuts="2">Picardie</region><settlement type="city">cedex</settlement></placeName></affiliation></author><author><name sortKey="Mansfield, Shawn D" sort="Mansfield, Shawn D" uniqKey="Mansfield S" first="Shawn D" last="Mansfield">Shawn D. Mansfield</name><affiliation wicri:level="4"><nlm:affiliation>Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada. shawn.mansfield@ubc.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, BC, V6T 1Z4</wicri:regionArea><orgName type="university">Université de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Campbell, Malcolm M" sort="Campbell, Malcolm M" uniqKey="Campbell M" first="Malcolm M" last="Campbell">Malcolm M. Campbell</name><affiliation wicri:level="4"><nlm:affiliation>Faculty of Forestry, University of Toronto, 33 Willcocks St., Toronto, ON, M5S 3B2, Canada. malcolm.campbell@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Faculty of Forestry, University of Toronto, 33 Willcocks St., Toronto, ON, M5S 3B2</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Centre for the Analysis of Genome Evolution and Function, University of Toronto, 25 Willcocks St., Toronto, ON, M5S 3B2, Canada. malcolm.campbell@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Centre for the Analysis of Genome Evolution and Function, University of Toronto, 25 Willcocks St., Toronto, ON, M5S 3B2</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Department of Cell & Systems Biology, University of Toronto, 25 Willcocks St., Toronto, ON, M5S 3B2, Canada. malcolm.campbell@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Cell & Systems Biology, University of Toronto, 25 Willcocks St., Toronto, ON, M5S 3B2</wicri:regionArea><orgName type="university">Université de Toronto</orgName><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Biological Sciences, University of Toronto Scarborough, Toronto, ON, M1C 1A4, Canada. malcolm.campbell@utoronto.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biological Sciences, University of Toronto Scarborough, Toronto, ON, M1C 1A4</wicri:regionArea><wicri:noRegion>M1C 1A4</wicri:noRegion></affiliation></author></analytic><series><title level="j">BMC genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cluster Analysis (MeSH)</term><term>Droughts (MeSH)</term><term>Energy Metabolism (genetics)</term><term>Gas Chromatography-Mass Spectrometry (MeSH)</term><term>Gene Regulatory Networks (MeSH)</term><term>Genotype (MeSH)</term><term>Metabolome (MeSH)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>RNA, Plant (analysis)</term><term>RNA, Plant (isolation & purification)</term><term>RNA, Plant (metabolism)</term><term>Time Factors (MeSH)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (analyse)</term><term>ARN des plantes (isolement et purification)</term><term>ARN des plantes (métabolisme)</term><term>Analyse de regroupements (MeSH)</term><term>Chromatographie gazeuse-spectrométrie de masse (MeSH)</term><term>Facteurs temps (MeSH)</term><term>Génotype (MeSH)</term><term>Métabolisme énergétique (génétique)</term><term>Métabolome (MeSH)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Réseaux de régulation génique (MeSH)</term><term>Sécheresses (MeSH)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Plant</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN des plantes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Energy Metabolism</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Métabolisme énergétique</term><term>Populus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Plant</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN des plantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN des plantes</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cluster Analysis</term><term>Droughts</term><term>Gas Chromatography-Mass Spectrometry</term><term>Gene Regulatory Networks</term><term>Genotype</term><term>Metabolome</term><term>Time Factors</term><term>Transcriptome</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de regroupements</term><term>Chromatographie gazeuse-spectrométrie de masse</term><term>Facteurs temps</term><term>Génotype</term><term>Métabolome</term><term>Réseaux de régulation génique</term><term>Sécheresses</term><term>Transcriptome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Drought has a major impact on tree growth and survival. Understanding tree responses to this stress can have important application in both conservation of forest health, and in production forestry. Trees of the genus Populus provide an excellent opportunity to explore the mechanistic underpinnings of forest tree drought responses, given the growing molecular resources that are available for this taxon. Here, foliar tissue of six water-deficit stressed P. balsamifera genotypes was analysed for variation in the metabolome in response to drought and time of day by using an untargeted metabolite profiling technique, gas chromatography/mass-spectrometry (GC/MS).</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Significant variation in the metabolome was observed in response the imposition of water-deficit stress. Notably, organic acid intermediates such as succinic and malic acid had lower concentrations in leaves exposed to drought, whereas galactinol and raffinose were found in increased concentrations. A number of metabolites with significant difference in accumulation under water-deficit conditions exhibited intraspecific variation in metabolite accumulation. Large magnitude fold-change accumulation was observed in three of the six genotypes. In order to understand the interaction between the transcriptome and metabolome, an integrated analysis of the drought-responsive transcriptome and the metabolome was performed. One P. balsamifera genotype, AP-1006, demonstrated a lack of congruence between the magnitude of the drought transcriptome response and the magnitude of the metabolome response. More specifically, metabolite profiles in AP-1006 demonstrated the smallest changes in response to water-deficit conditions.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>Pathway analysis of the transcriptome and metabolome revealed specific genotypic responses with respect to primary sugar accumulation, citric acid metabolism, and raffinose family oligosaccharide biosynthesis. The intraspecific variation in the molecular strategies that underpin the responses to drought among genotypes may have an important role in the maintenance of forest health and productivity.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25895923</PMID><DateCompleted><Year>2016</Year><Month>01</Month><Day>27</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2164</ISSN><JournalIssue CitedMedium="Internet"><Volume>16</Volume><PubDate><Year>2015</Year><Month>Apr</Month><Day>21</Day></PubDate></JournalIssue><Title>BMC genomics</Title><ISOAbbreviation>BMC Genomics</ISOAbbreviation></Journal><ArticleTitle>Poplar trees reconfigure the transcriptome and metabolome in response to drought in a genotype- and time-of-day-dependent manner.</ArticleTitle><Pagination><MedlinePgn>329</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s12864-015-1535-z</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Drought has a major impact on tree growth and survival. Understanding tree responses to this stress can have important application in both conservation of forest health, and in production forestry. Trees of the genus Populus provide an excellent opportunity to explore the mechanistic underpinnings of forest tree drought responses, given the growing molecular resources that are available for this taxon. Here, foliar tissue of six water-deficit stressed P. balsamifera genotypes was analysed for variation in the metabolome in response to drought and time of day by using an untargeted metabolite profiling technique, gas chromatography/mass-spectrometry (GC/MS).</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Significant variation in the metabolome was observed in response the imposition of water-deficit stress. Notably, organic acid intermediates such as succinic and malic acid had lower concentrations in leaves exposed to drought, whereas galactinol and raffinose were found in increased concentrations. A number of metabolites with significant difference in accumulation under water-deficit conditions exhibited intraspecific variation in metabolite accumulation. Large magnitude fold-change accumulation was observed in three of the six genotypes. In order to understand the interaction between the transcriptome and metabolome, an integrated analysis of the drought-responsive transcriptome and the metabolome was performed. One P. balsamifera genotype, AP-1006, demonstrated a lack of congruence between the magnitude of the drought transcriptome response and the magnitude of the metabolome response. More specifically, metabolite profiles in AP-1006 demonstrated the smallest changes in response to water-deficit conditions.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">Pathway analysis of the transcriptome and metabolome revealed specific genotypic responses with respect to primary sugar accumulation, citric acid metabolism, and raffinose family oligosaccharide biosynthesis. The intraspecific variation in the molecular strategies that underpin the responses to drought among genotypes may have an important role in the maintenance of forest health and productivity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hamanishi</LastName><ForeName>Erin T</ForeName><Initials>ET</Initials><AffiliationInfo><Affiliation>Faculty of Forestry, University of Toronto, 33 Willcocks St., Toronto, ON, M5S 3B2, Canada. erin.hamanishi@utoronto.ca.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Centre for the Analysis of Genome Evolution and Function, University of Toronto, 25 Willcocks St., Toronto, ON, M5S 3B2, Canada. erin.hamanishi@utoronto.ca.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Barchet</LastName><ForeName>Genoa L H</ForeName><Initials>GL</Initials><AffiliationInfo><Affiliation>Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada. genoalily@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dauwe</LastName><ForeName>Rebecca</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada. rebecca.dauwe@u-picardie.fr.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Current address: Département des sciences agronomiques et écologiques, Université de Picardie Jules Verne, Amiens, 80039 cedex, France. rebecca.dauwe@u-picardie.fr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mansfield</LastName><ForeName>Shawn D</ForeName><Initials>SD</Initials><AffiliationInfo><Affiliation>Department of Wood Science, University of British Columbia, 4030-2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada. shawn.mansfield@ubc.ca.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Campbell</LastName><ForeName>Malcolm M</ForeName><Initials>MM</Initials><AffiliationInfo><Affiliation>Faculty of Forestry, University of Toronto, 33 Willcocks St., Toronto, ON, M5S 3B2, Canada. malcolm.campbell@utoronto.ca.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Centre for the Analysis of Genome Evolution and Function, University of Toronto, 25 Willcocks St., Toronto, ON, M5S 3B2, Canada. malcolm.campbell@utoronto.ca.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Cell & Systems Biology, University of Toronto, 25 Willcocks St., Toronto, ON, M5S 3B2, Canada. malcolm.campbell@utoronto.ca.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biological Sciences, University of Toronto Scarborough, Toronto, ON, M1C 1A4, Canada. malcolm.campbell@utoronto.ca.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>04</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Genomics</MedlineTA><NlmUniqueID>100965258</NlmUniqueID><ISSNLinking>1471-2164</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016000" MajorTopicYN="N">Cluster Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055864" MajorTopicYN="N">Droughts</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004734" MajorTopicYN="N">Energy Metabolism</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008401" MajorTopicYN="N">Gas Chromatography-Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D053263" MajorTopicYN="N">Gene Regulatory Networks</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055442" MajorTopicYN="Y">Metabolome</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time 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Hamanishi</name></region><name sortKey="Barchet, Genoa L H" sort="Barchet, Genoa L H" uniqKey="Barchet G" first="Genoa L H" last="Barchet">Genoa L H. Barchet</name><name sortKey="Campbell, Malcolm M" sort="Campbell, Malcolm M" uniqKey="Campbell M" first="Malcolm M" last="Campbell">Malcolm M. Campbell</name><name sortKey="Campbell, Malcolm M" sort="Campbell, Malcolm M" uniqKey="Campbell M" first="Malcolm M" last="Campbell">Malcolm M. Campbell</name><name sortKey="Campbell, Malcolm M" sort="Campbell, Malcolm M" uniqKey="Campbell M" first="Malcolm M" last="Campbell">Malcolm M. Campbell</name><name sortKey="Campbell, Malcolm M" sort="Campbell, Malcolm M" uniqKey="Campbell M" first="Malcolm M" last="Campbell">Malcolm M. Campbell</name><name sortKey="Dauwe, Rebecca" sort="Dauwe, Rebecca" uniqKey="Dauwe R" first="Rebecca" last="Dauwe">Rebecca Dauwe</name><name sortKey="Hamanishi, Erin T" sort="Hamanishi, Erin T" uniqKey="Hamanishi E" first="Erin T" last="Hamanishi">Erin T. Hamanishi</name><name sortKey="Mansfield, Shawn D" sort="Mansfield, Shawn D" uniqKey="Mansfield S" first="Shawn D" last="Mansfield">Shawn D. Mansfield</name></country><country name="France"><region name="Hauts-de-France"><name sortKey="Dauwe, Rebecca" sort="Dauwe, Rebecca" uniqKey="Dauwe R" first="Rebecca" last="Dauwe">Rebecca Dauwe</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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